Immunoinformatic analysis of glycoprotein from bovine ephemeral fever virus

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cloning and sequencing of g glycoprotein gene of bovine ephemeral fever virus in escherichia coli

introduction bovine ephemeral fever (bef) is an arthropod-borne viral disease of cattle and water buffalo, spanning tropical and subtropical zones in asia, australia, and africa continents. the clinical signs of bef disease in cattle are acute febrile reaction, stiffness, lameness, depression, cessation of rumination, and constipation. this disease was caused by ephemerovirus of the rhabdovirid...

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DNA sequence analysis of glycoprotein G gene of bovine ephemeral fever virus and development of a double oil emulsion vaccine against bovine ephemeral fever.

The surface glycoprotein G is considered as the major neutralizing and protective antigen of bovine ephemeral fever virus (BEFV). Comparison of the deduced amino acid sequence of G protein of BEFV isolates during the period 1984-2004 outbreaks in Taiwan showed amino acid substitutions in the neutralizing epitopes. All the isolates differ markedly in the neutralizing epitope at the same amino ac...

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Production of Monoclonal Antibody against Prokaryotically Expressed G1 Protein of Bovine Ephemeral Fever Virus

Epitope-G1 of bovine ephemeral fever virus (BEFV) G glycoprotein has been genetically and antigenically conserved among various isolates of BEFV and only reacts with anti-BEFV neutralising antibodies. Therefore, it is a candidate antigen for development of the enzyme linked immunosorbent assay (ELISA) for serological identification bovine ephemeral fever (BEF)-infected animals. The aim of this ...

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Evolution of bovine ephemeral fever virus in the Australian episystem.

Bovine ephemeral fever virus (BEFV) is an arthropod-borne rhabdovirus that causes a debilitating disease of cattle in Africa, Asia, and Australia; however, its global geodynamics are poorly understood. An evolutionary analysis of G gene (envelope glycoprotein) ectodomain sequences of 97 BEFV isolates collected from Australia during 1956 to 2012 revealed that all have a single common ancestor an...

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ژورنال

عنوان ژورنال: Biomedical and Biotechnology Research Journal (BBRJ)

سال: 2018

ISSN: 2588-9834

DOI: 10.4103/bbrj.bbrj_71_18